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KMID : 1195620150080010083
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Clinical and Experimental Otorhinolaryngology
2015 Volume.8 No. 1 p.83 ~ p.91
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Neural-Induced Human Mesenchymal Stem Cells Promote Cochlear Cell Regeneration in Deaf Guinea Pigs
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Jang Su-Jeong
Cho Hyong-Ho
Kim Song-Hee
Lee Kyung-Hwa
Jun Jae-Yeoul
Park Jong-Seong
Jeong Han-Seong
Cho Yong-Beom
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Abstract
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Objectives: In mammals, cochlear hair cell loss is irreversible and may result in a permanent sensorineural hearing loss. Secondary to this hair cell loss, a progressive loss of spiral ganglion neurons (SGNs) is presented. In this study, we have investigated the effects of neural-induced human mesenchymal stem cells (NI-hMSCs) from human bone marrow on sensory neuronal regeneration from neomycin treated deafened guinea pig cochleae.
Methods: HMSCs were isolated from the bone marrow which was obtained from the mastoid process during mastoidectomy for ear surgery. Following neural induction with basic fibroblast growth factor and forskolin, we studied the several neural marker and performed electrophysiological analysis. NI-hMSCs were transplanted into the neomycin treated deafened guinea pig cochlea. Engraftment of NI-hMSCs was evaluated immunohistologically at 8 weeks after transplantation.
Results: Following neural differentiation, hMSCs expressed high levels of neural markers, ionic channel markers, which are important in neural function, and tetrodotoxin-sensitive voltage-dependent sodium currents. After transplantation into the scala tympani of damaged cochlea, NI-hMSCs-injected animals exhibited a significant increase in the number of SGNs compared to Hanks balanced salt solution-injected animals. Transplanted NI-hMSCs were found within the perilymphatic space, the organ of Corti, along the cochlear nerve fibers, and in the spiral ganglion. Furthermore, the grafted NI-hMSCs migrated into the spiral ganglion where they expressed the neuron-specific marker, NeuN.
Conclusion: The results show the potential of NI-hMSCs to give rise to replace the lost cochlear cells in hearing loss mammals.
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KEYWORD
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Hair Cells, Auditory, Mesenchymal Stem Cells, Hearing Loss, Cell Differentiation, Transplantation
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